Pill to prevent listeriosis may be on the horizon

Modified probiotics, the beneficial bacteria touted for their role in digestive health, could one day decrease the risk of Listeria infection in people with susceptible immune systems, according to Purdue University research.

Arun Bhunia, a professor of food science; Mary Anne Amalaradjou, a Purdue postdoctoral researcher; and Ok Kyung Koo, a former Purdue doctoral student, found that the same Listeria protein that allows the bacteria to pass through intestinal cells and into bloodstreams can help block those same paths when added to a probiotic.

“Based on the research, it looks very promising that we would get a significant reduction in Listeria infections,” said Bhunia, whose findings were published in the journal PLoS One in January.

Bhunia’s earlier work showed that Listeria triggers intestinal cells to express heat shock protein 60 on their surfaces. That allows Listeria to bind to the intestinal cells using an adhesion protein and pass into them, acting as a sort of gateway to the bloodstream.

Once in the bloodstream, even small doses of Listeria can cause fever, muscle aches, nausea and diarrhoea, as well as headaches, stiff neck, confusion, loss of balance and convulsions if it spreads to the nervous system. It can also cause abortion and stillbirth in pregnant women.

According to the US Centers for Disease Control and Prevention, it sickens about 1500 and kills 255 people each year in the United States and primarily affects pregnant women, newborns, older adults and those with weakened immune systems.

“We’re seeing fewer Listeria infections, but the severity of those infections is still high,” Amalaradjou said.

The researchers found that probiotics alone were ineffective in combatting Listeria, so they stole a trick from the bacteria’s own playbook. By adding the Listeria adhesion protein to the probiotic Lactobacillus paracasei, they were able to decrease the number of Listeria cells that passed through intestinal cells by 46%, a significant decrease in the amount of the bacteria that could infect a susceptible person.

With the adhesion protein, Lactobacillus paracasei interacts with heat shock protein on the surface of intestinal cells just as Listeria would. The probiotic then attached to the intestinal cells, crowding out Listeria.

“It’s creating a competition,” Bhunia said. “If Listeria comes in, it doesn’t find a place to attach or invade.”

Bhunia said he could one day foresee the development of a pill or probiotic drink that could be given to at-risk patients to minimise the risk of Listeria infection.