Oil-water separation with biofilm solution

A type of biofilm made by bacteria could become a useful tool in cleaning contaminated water.

Researchers from North Carolina State University (NC State) have discovered that certain bacteria make a slimy but tough biofilm for protection and motility — and it can also be used to separate water and oil.

The researchers used the material produced by the bacteria Gluconacetobacter hansenii as a filter to separate water from an oil mixture, with results published in the journal Langmuir.

“It’s really remarkable to think that these little bugs can make this stuff that is so perfect in many ways,” said Lucian Lucia, the study’s corresponding author and an Associate Professor of Forest Biomaterials And Chemistry at NC State.

The biofilm the bacteria make and release into their environment is made of cellulose — the same material that gives plants a sturdy structure in their cell walls. However, when bacteria make cellulose, it has a tightly packed, crystalline structure.

“It’s one of the purest, if not the purest, forms of cellulose out there,” Assoc Prof Lucia said. “It’s very well structured. It’s very water-loving, and it’s got a very high crystallinity, so it packs very beautifully. Once you strip out the bacteria, you have this amazingly tough material that has a real robustness, or toughness.”

The researchers explained that the bacteria make the film to protect themselves.

“If you leave something like an unwashed dish out, it can turn all slimy and gross — that’s a biofilm,” said study co-author Wendy Krause, Associate Professor of Textile Engineering, Chemistry And Science at NC State. “Different bacteria make different biofilms. The bacterial film that we’re studying is made of cellulose. The bacteria are making it because they live on it and in it. They’re making their home.”

In the experiment, researchers used the bacteria as factories of cellulose nanofibres, then removed the bacteria and their non-cellulose residue. Finally, the researchers used the cellulose membrane to see if it could separate water from a solution containing oil and water, finding that the material was sturdy and effective at removing water.

“The oil doesn’t want to go through the membrane; it has a repulsive effect to it,” Assoc Prof Lucia said. “It’s super fat-hating.”

“If the oil and water were highly mixed, it doesn’t matter,” Assoc Prof Krause added. “You could put an immersion blender into the solution, and the membrane will still separate the water and oil.”

The researchers see a variety of potential applications for the material, such as to recover water from an oily mixture. In future work, the researchers want to explore how they can tailor the membrane by chemically modifying it for certain applications.

Image credit: ©stock.adobe.com/au/magann