Dionex App Note 270: Determination of Hydroxymethylfurfural in Honey and Biomass

Dionex has published a new high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD)-based method for the determination of hydroxymethylfurfural (HMF) in samples ranging from food (honey and pancake syrup) to treated biomass (corn stover and wood hydrolysate). HMF is naturally found in many foods (eg, honey) and can increase during cooking or processing. The concentration of HMF can indicate food quality and authenticity and some countries have proposed limits to its content in food.

Application Note 270: Determination of Hydroxymethylfurfural in Honey and Biomass demonstrates that this method has a broad linear range, high precisions and low detection limits. This system configuration requires only addition of deionised water for continuous operation. This application note describes the use of a Dionex ICS-3000 system with a CarboPac PA1 column, electrolytically generated hydroxide eluent and electrochemical detection with a disposable Au-on-polytetrafluoroethylene (PTFE) working electrode. The CarboPac PA1 is a high-capacity, rugged column suitable for determining mono and disaccharides and has high resolution for HMF in a wide variety of matrices. The disposable Au working electrode provides consistently high detector response, assuring greater instrument-to-instrument and lab-to-lab reproducibility. The HPAE-PAD-based HMF analysis method described in AN 270 is accurate, reliable and applicable to online monitoring of HMF levels in food and can be found at www.dionex.com under the Documents tab.