Hunting bacteria

A fluorescent test system has been developed that finds specific pathogens by tracking their spoor.

They can be in our water and in our food, multiplying so rapidly that conventional testing methods for detecting pathogens such as E. coli, Salmonella and Listeria come too late for the tens of thousands of people who suffer the ill effects of these potentially deadly bacteria.

Biochemist Yingfu Li from the Faculty of Health Sciences at McMaster University in Canada and his research team have developed a simple test that can swiftly and accurately identify specific pathogens using a system that will ‘hunt’ for bacteria, identifying their harmful presence before they have a chance to contaminate food and water.

Like any living thing, bacteria have their own spoor, leaving behind DNA trails of bacterial ‘droppings’. Li tracks these metabolic by-products with molecular beacons - little lighthouses on a molecular scale that actually light up when they detect the DNA sequence left behind.

Li created a DNAzyme sensor that will be able to identify any bacteria, utilising a method that doesn’t require the steps and specialised equipment typically used to identify whether or not harmful bacteria are present.

“Current methods of foodborne bacterial detection take time. The five days it takes to detect Listeria, for example, can translate into an outbreak that costs lives. We have developed a universal test that uses less complex procedures but still generates precise and accurate results,” says Li, a Canada Research Chair in Directed Evolution of Nucleic Acids.

Li’s fluorescent test system was highlighted in Angewandte Chemie International Edition, a prestigious weekly chemistry journal that ranks among the best for the original research it publishes. Li’s paper, co-authored with lab members Monsur Ali, Sergio Aguirre and Hadeer Lazim, was designated a ‘hot paper’ by Angewandte’s editors for its “importance in a rapidly evolving field of current interest”.

“McMaster researchers are known for their ability to provide solutions to problems that impact the public’s wellbeing. The test that Professor Li has developed will help safeguard the health of consumers and supply industry with a reliable means to bring safe food products to consumers and reduce their time to market,” said Mo Elbestawi, Vice-President, Research And International Affairs.

Li’s research was funded by the Natural Sciences and Engineering Research Council (NSERC) and the Sentinel Bioactive Paper Network.

For a full copy of the paper, visit: http://onlinelibrary.wiley.com/doi/10.1002/anie.201100477/full

For more information, please contact:

Professor Yingfu Li

Associate Professor, Dept. of Biochemistry & Biomedical Sciences and

Canada Research Chair in Directed Evolution of Nucleic Acids

liying@mcmaster.ca